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Author

Kang, Sebyung
Protein Nanobio Lab
Research Interests
  • Protein engineering, Drug/diagnostics delivery nanoplatform, Protein-base vaccine delivery systems, Biosensor & imaging

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Targeted Delivery of a Photosensitizer to Aggregatibacter actinomycetemcomitans Biofilm

Cited 11 times inthomson ciCited 11 times inthomson ci
Title
Targeted Delivery of a Photosensitizer to Aggregatibacter actinomycetemcomitans Biofilm
Author
Suci, PeterKang, SebyungGmuer, RudolfDouglas, TrevorYoung, Mark
Keywords
PROTEIN CAGE ARCHITECTURE; RESISTANT STAPHYLOCOCCUS-AUREUS; PHOTODYNAMIC THERAPY; ACTINOBACILLUS-ACTINOMYCETEMCOMITANS; LETHAL PHOTOSENSITIZATION; AGGRESSIVE PERIODONTITIS; MONOCLONAL-ANTIBODIES; ANTIMICROBIAL PEPTIDE; CLINICAL-SAMPLES; ORAL INFECTIONS
Issue Date
201006
Publisher
AMER SOC MICROBIOLOGY
Citation
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, v.54, no.6, pp.2489 - 2496
Abstract
The ability to selectively target specific biofilm species with antimicrobials would enable control over biofilm consortium composition, with medical applications in treatment of infections on mucosal surfaces that are colonized by a mixture of beneficial and pathogenic microorganisms. We functionalized a genetically engineered multimeric protein with both a targeting moiety (biotin) and either a fluorophore or a photosensitizer (SnCe6). Biofilm microcolonies of Aggregatibacter actinomycetemcomitans, a periodontal pathogen, were targeted with the multifunctional dodecamer. Streptavidin was used to couple biotinylated dodecamer to a biotinylated anti-A. actinomycetemcomitans antibody. This modular targeting approach enabled us to increase the loading of photosensitizer onto the cells by a cycle of amplification. Scanning laser confocal microscopy was used to characterize transport of fluorescently tagged dodecamer into the microcolonies and targeting of the cells with biotin-labeled, fluorescently tagged dodecamer. Light-induced activity of the targeted photosensitizer reduced the viability of A. actinomycetemcomitans biofilm, as indicated by membrane permeability to propidium iodide. The functionalized multimeric protein promises to be a useful tool for controlling periodontal biofilm consortia and offers a modular design whereby moieties that target different species can be readily combined with the functionalized protein construct.
URI
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DOI
http://dx.doi.org/10.1128/AAC.00059-10
ISSN
0066-4804
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SLS_Journal Papers
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