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Jang, Jaesung
Sensors & Aerosols Lab.
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Rapid and selective concentration of bacteria, viruses, and proteins using alternating current signal superimposition on two coplanar electrodes

Author(s)
Han, Chang-HoWoo, Seong YongBhardwaj, JyotiSharma, AbhinavJang, Jaesung
Issued Date
2018-10
DOI
10.1038/s41598-018-33329-7
URI
https://scholarworks.unist.ac.kr/handle/201301/24938
Fulltext
https://www.nature.com/articles/s41598-018-33329-7
Citation
SCIENTIFIC REPORTS, v.8, pp.14942
Abstract
Dielectrophoresis (DEP) is usually effective close to the electrode surface. Several techniques have been developed to overcome its drawbacks and to enhance dielectrophoretic particle capture. Here we present a simple technique of superimposing alternating current DEP (high-frequency signals) and electroosmosis (EO; low-frequency signals) between two coplanar electrodes (gap: 25 mu m) using a lab-made voltage adder for rapid and selective concentration of bacteria, viruses, and proteins, where we controlled the voltages and frequencies of DEP and EO separately. This signal superimposition technique enhanced bacterial capture (Escherichia coli K-12 against 1-mu m-diameter polystyrene beads) more selectively (>99%) and rapidly (similar to 30 s) at lower DEP (5 Vpp) and EO (1.2 Vpp) potentials than those used in the conventional DEP capture studies. Nanometer-sized MS2 viruses and troponin I antibody proteins were also concentrated using the superimposed signals, and significantly more MS2 and cTnI-Ab were captured using the superimposed signals than the DEP (10 Vpp) or EO (2 Vpp) signals alone (p < 0.035) between the two coplanar electrodes and at a short exposure time (1 min). This technique has several advantages, such as simplicity and low cost of electrode fabrication, rapid and large collection without electrolysis.
Publisher
NATURE PUBLISHING GROUP
ISSN
2045-2322
Keyword
AC ELECTROOSMOTIC MICROMIXERINDUCED FLUID-FLOWDIELECTROPHORETIC CONCENTRATIONSUBMICRON BIOPARTICLESSEPARATIONMANIPULATIONCELLSMEDIABACTERIOPHAGESPARTICLES

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