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dc.citation.endPage 97 -
dc.citation.number 1 -
dc.citation.startPage 87 -
dc.citation.title ANALYST -
dc.citation.volume 137 -
dc.contributor.author Emory, Jason M. -
dc.contributor.author Peng, Zhiyong -
dc.contributor.author Young, Brandon -
dc.contributor.author Hupert, Mateusz L. -
dc.contributor.author Rousselet, Arnold -
dc.contributor.author Patterson, Donald -
dc.contributor.author Ellison, Brad -
dc.contributor.author Soper, Steven A. -
dc.date.accessioned 2023-12-22T05:37:28Z -
dc.date.available 2023-12-22T05:37:28Z -
dc.date.created 2013-06-12 -
dc.date.issued 2012-01 -
dc.description.abstract Single-molecule detection (SMD) has demonstrated some attractive benefits for many types of biomolecular analyses including enhanced processing speed by eliminating processing steps, elimination of ensemble averaging and single-molecule sensitivity. However, it's wide spread use has been hampered by the complex instrumentation required for its implementation when using fluorescence as the readout modality. We report herein a simple and compact fluorescence single-molecule instrument that is straightforward to operate and consisted of fiber optics directly coupled to a microfluidic device. The integrated fiber optics served as waveguides to deliver the laser excitation light to the sample and collecting the resulting emission, simplifying the optical requirements associated with traditional SMD instruments by eliminating the need for optical alignment and simplification of the optical train. Additionally, the use of a vertical cavity surface emitting laser and a single photon avalanche diode serving as the excitation source and photon transducer, respectively, as well as a field programmable gate array (FPGA) integrated into the processing electronics assisted in reducing the instrument footprint. This small footprint SMD platform was tested using fluorescent microspheres and single AlexaFluor 660 molecules to determine the optimal operating parameters and system performance. As a demonstration of the utility of this instrument for biomolecular analyses, molecular beacons (MBs) were designed to probe bacterial cells for the gene encoding Gram-positive species. The ability to monitor biomarkers using this simple and portable instrument will have a number of important applications, such as strain-specific detection of pathogenic bacteria or the molecular diagnosis of diseases requiring rapid turn-around-times directly at the point-of-use. -
dc.identifier.bibliographicCitation ANALYST, v.137, no.1, pp.87 - 97 -
dc.identifier.doi 10.1039/c1an15658f -
dc.identifier.issn 0003-2654 -
dc.identifier.scopusid 2-s2.0-82555181625 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/2457 -
dc.identifier.url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=82555181625 -
dc.identifier.wosid 000298617500012 -
dc.language 영어 -
dc.publisher ROYAL SOC CHEMISTRY -
dc.title Design and development of a field-deployable single-molecule detector (SMD) for the analysis of molecular markers -
dc.type Article -
dc.relation.journalWebOfScienceCategory Chemistry, Analytical -
dc.relation.journalResearchArea Chemistry -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -

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