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Takayama, Shuichi
Cell and Microfluidics Lab
Research Interests
  • Bio-MEMS and Microfluidics

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Micro- and nanofluidic technologies for epigenetic profiling

Cited 4 times inthomson ciCited 1 times inthomson ci
Title
Micro- and nanofluidic technologies for epigenetic profiling
Author
Matsuoka, ToshikiKim, Byoung ChoulMoraes, ChristopherHan, MinsubTakayama, Shuichi
Keywords
DNA; genetics; genomics; microfluidics; nanofluidics; reviews
Issue Date
201307
Publisher
AMER INST PHYSICS
Citation
BIOMICROFLUIDICS, v.7, no.4, pp.1 - 11
Abstract
This short review provides an overview of the impact micro- and nanotechnologies can make in studying epigenetic structures. The importance of mapping histone modifications on chromatin prompts us to highlight the complexities and challenges associated with histone mapping, as compared to DNA sequencing. First, the histone code comprised over 30 variations, compared to 4 nucleotides for DNA. Second, whereas DNA can be amplified using polymerase chain reaction, chromatin cannot be amplified, creating challenges in obtaining sufficient material for analysis. Third, while every person has only a single genome, there exist multiple epigenomes in cells of different types and origins. Finally, we summarize existing technologies for performing these types of analyses. Although there are still relatively few examples of micro- and nanofluidic technologies for chromatin analysis, the unique advantages of using such technologies to address inherent challenges in epigenetic studies, such as limited sample material, complex readouts, and the need for high-content screens, make this an area of significant growth and opportunity
URI
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DOI
http://dx.doi.org/10.1063/1.4816835
ISSN
1932-1058
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SLS_Journal Papers
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