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Author

Kang, Joo H.
Translational Multiscale Biofluidics Lab (TMB Lab)
Research Interests
  • Biomedical devices, infectious disease, organ-on-a-chip, microfluidics, mechanobiology

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Magnetic activated cell sorting (MACS) pipette tip for immunomagnetic bacteria separation

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Title
Magnetic activated cell sorting (MACS) pipette tip for immunomagnetic bacteria separation
Author
Oh, SeinJung, Su HyunSeo, HyekyungMin, Mun-KyungKim, ByeongyeonHahn, Young KiKang, Joo H.Choi, Sungyoung
Keywords
Magnetic activated cell sorting;  Pipette tip;  Immunomagnetic separation;  Microfluidics;  Bacteria separation
Issue Date
201811
Publisher
ELSEVIER SCIENCE SA
Citation
SENSORS AND ACTUATORS B-CHEMICAL, v.272, no., pp.324 - 330
Abstract
Magnetic activated cell sorting is a well-established technology to sort target cells from heterogeneous cell populations based on their specific surface receptors. However, conventional bulk separators rely on irregular and dense ferromagnetic matrices that can cause variances in separation purity and recovery. Recent microfluidic separators typically use narrow and two-dimensional laminar flows that can lead to low separation throughput. Here, we present a magnetizable micropipette tip incorporating a ferromagnetic matrix of nickel meshes that have regular and rectangular micropores. The functional pipette tip can be transiently magnetized by permanent magnets, thereby enabling the efficient capture and release of target cells by simple pipetting. We performed parametric studies to investigate optimal separation protocols such as the number of mesh layers and pipetting repetitions. We then applied the functional pipette tip for separation of bacterial cells from whole blood, confirming that the separation quality is sufficient for downstream cell culture and analysis. The magnetizable pipette tip provides high-throughput separation, processing 1 mL of blood within 7 min, and high-recovery separation, recovering ≈90.5% of the bacterial cells spiked into blood.
URI
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DOI
http://dx.doi.org/10.1016/j.snb.2018.05.146
ISSN
0925-4005
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