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Schultz, Thomas
CRASY(Correlated Rotational Alignment Spectroscopy)
Research Interests
  • Correlated spectroscopy

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Femtosecond laser-induced dissociation (fs-LID) as an activation method in mass spectrometry

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Title
Femtosecond laser-induced dissociation (fs-LID) as an activation method in mass spectrometry
Author
Neidel, ChristianKuehn, AndreasSchulz, Claus PeterHertel, Ingolf V.Linscheid, Michael W.Schultz, Thomas
Keywords
Fs-LID; Peptides; Proteins; Sequencing; Mass spectrometry; FT-MS; Femtosecond laser
Issue Date
201805
Publisher
ELSEVIER SCIENCE BV
Citation
CHEMICAL PHYSICS, v.514, no., pp.106 - 112
Abstract
We report the photolysis of biomolecules in a Fourier-transform ion cyclotron resonance mass spectrometer by intense near-infrared femtosecond laser pulses. Photo-fragmentation was accompanied by photo-ionization and created a large number and variety of charged fragments, which could be identified with high confidence due to the outstanding resolving power and mass accuracy of the mass spectrometer. Fragmentation patterns were sufficient for peptide sequence analysis. Fragments formed by non-ergodic cleavage retained labile post-translational modifications.
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DOI
http://dx.doi.org/10.1016/j.chemphys.2018.05.008
ISSN
0301-0104
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