Carbonic anhydrase II microcrystals suitable for XFEL studies
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- Carbonic anhydrase II microcrystals suitable for XFEL studies
- Lomelino, Carrie L.; Kim, Jin Kyun; Lee, Cheol; Lim, Seon Woo; Andring, Jacob T.; Mahon, Brian P.; Chung, Moses; Kim, Chae Un; McKenna, R
- X-ray free-electron lasers; XFELs; serial femtosecond crystallography; time-resolved crystallography; microcrystals; carbonic anhydrase II.
- Issue Date
- INT UNION CRYSTALLOGRAPHY
- ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, v.F74, no.6, pp.327 - 330
- Recent advances in X-ray free-electron laser (XFEL) sources have permitted the study of protein dynamics. Femtosecond X-ray pulses have allowed the visualization of intermediate states in enzyme catalysis. In this study, the growth of carbonic anhydrase II microcrystals (40–80 µm in length) suitable for the collection of XFEL diffraction data at the Pohang Accelerator Laboratory is demonstrated. The crystals diffracted to 1.7 Å resolution and were indexed in space group P21, with unit-cell parameters a = 42.2, b = 41.2, c = 72.0 Å, β = 104.2°. These preliminary results provide the necessary framework for time-resolved experiments to study carbonic anhydrase catalysis at XFEL beamlines.
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