BROWSE

Related Researcher

Author

Kim, Chae Un
High Pressure X-ray Science Lab (HiPreX)
Research Interests
  • X-ray Science, biophysical science, biomedical science

ITEM VIEW & DOWNLOAD

Carbonic anhydrase II microcrystals suitable for XFEL studies

Cited 0 times inthomson ciCited 0 times inthomson ci
Title
Carbonic anhydrase II microcrystals suitable for XFEL studies
Author
Lomelino, Carrie L.Kim, Jin KyunLee, CheolLim, Seon WooAndring, Jacob T.Mahon, Brian P.Chung, MosesKim, Chae UnMcKenna, R
Keywords
X-ray free-electron lasers; XFELs; serial femtosecond crystallography; time-resolved crystallography; microcrystals; carbonic anhydrase II.
Issue Date
201806
Publisher
INT UNION CRYSTALLOGRAPHY
Citation
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, v.F74, no.6, pp.327 - 330
Abstract
Recent advances in X-ray free-electron laser (XFEL) sources have permitted the study of protein dynamics. Femtosecond X-ray pulses have allowed the visualization of intermediate states in enzyme catalysis. In this study, the growth of carbonic anhydrase II microcrystals (40–80 µm in length) suitable for the collection of XFEL diffraction data at the Pohang Accelerator Laboratory is demonstrated. The crystals diffracted to 1.7 Å resolution and were indexed in space group P21, with unit-cell parameters a = 42.2, b = 41.2, c = 72.0 Å, β = 104.2°. These preliminary results provide the necessary framework for time-resolved experiments to study carbonic anhydrase catalysis at XFEL beamlines.
URI
Go to Link
DOI
http://dx.doi.org/10.1107/S2053230X18006118
ISSN
2053-230X
Appears in Collections:
SNS_Journal Papers

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qr_code

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU