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Kim, Yong Hwan
School of Energy and Chemical Engineering
Research Interests
  • 바이오촉매 생물 전환 생물환경

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Bioelectrochemical conversion of CO2 to value added product formate using engineered Methylobacterium extorquens

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Title
Bioelectrochemical conversion of CO2 to value added product formate using engineered Methylobacterium extorquens
Author
Jang, JunghoJeon, Byoung WookKim, Yong Hwan
Issue Date
201805
Publisher
NATURE PUBLISHING GROUP
Citation
SCIENTIFIC REPORTS, v.8, no., pp.7211 -
Abstract
The conversion of carbon dioxide to formate is a fundamental step for building C1 chemical platforms. Methylobacterium extorquens AM1 was reported to show remarkable activity converting carbon dioxide into formate. Formate dehydrogenase 1 from M. extorquens AM1 (MeFDH1) was verified as the key responsible enzyme for the conversion of carbon dioxide to formate in this study. Using a 2% methanol concentration for induction, microbial harboring the recombinant MeFDH1 expressing plasmid produced the highest concentration of formate (26.6 mM within 21 hours) in electrochemical reactor. 60 μM of sodium tungstate in the culture medium was optimal for the expression of recombinant MeFDH1 and production of formate (25.7 mM within 21 hours). The recombinant MeFDH1 expressing cells showed maximum formate productivity of 2.53 mM/g-wet cell/hr, which was 2.5 times greater than that of wild type. Thus, M. extorquens AM1 was successfully engineered by expressing MeFDH1 as recombinant enzyme to elevate the production of formate from CO2 after elucidating key responsible enzyme for the conversion of CO2 to formate.
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DOI
http://dx.doi.org/10.1038/s41598-018-23924-z
ISSN
2045-2322
Appears in Collections:
ECHE_Journal Papers
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