This paper proposes the design of microstrip patch antennas for dual-band polarization adjustment. The antenna has a multi-layer structure for dual-band operation, and each layer contains a resonating patch with surrounding strips separated into two parts. The antenna polarization is adjusted by varying the separated positions of the strips, while fixing other design parameters. To demonstrate the feasibility, an antenna sample with right-hand circular polarization is fabricated, and its antenna characteristics are measured in a full anechoic chamber. The operating principle of polarization adjustment in the dual frequency bands is also verified by observing near electromagnetic fields and the magnetic surface current density around the antenna.