BROWSE

Related Researcher

Author

Mitchell, Robert J.
Applied & Environmental Microbiology Lab (AEML)
Research Interests
  • Pathogens, bdellovibrio bacteriovorus, patho-biotechnology

ITEM VIEW & DOWNLOAD

Combined Application of Bacterial Predation and Violacein to Kill Polymicrobial Pathogenic Communities

Cited 0 times inthomson ciCited 0 times inthomson ci
Title
Combined Application of Bacterial Predation and Violacein to Kill Polymicrobial Pathogenic Communities
Author
Im, HansolChoi, Seong YeolSon, SangmoMitchell, Robert J.
Issue Date
201712
Publisher
NATURE PUBLISHING GROUP
Citation
SCIENTIFIC REPORTS, v.7, no., pp.14415 -
Abstract
Violacein is a bisindole antibiotic that is effective against Gram-positive bacteria while the bacterial predator, Bdellovibrio bacteriovorus HD100, predates on Gram-negative strains. In this study, we evaluated the use of both together against multidrug resistant pathogens. The two antibacterial agents did not antagonize the activity of the other. For example, treatment of Staphylococcus aureus with violacein reduced its viability by more than 2,000-fold with or without B. bacteriovorus addition. Likewise, predation of Acinetobacter baumannii reduced the viability of this pathogen by more than 13,000-fold, regardless if violacein was present or not. When used individually against mixed bacterial cultures containing both Gram-positive and Gram-negative strains, violacein and B. bacteriovorus HD100 were effective against only their respective strains. The combined application of both violacein and B. bacteriovorus HD100, however, reduced the total pathogen numbers by as much as 84,500-fold. Their combined effectiveness was also demonstrated using a 4-species culture containing S. aureus, A. baumannii, Bacillus cereus and Klebsiella pneumoniae. When used alone, violacein and bacterial predation reduced the total population by only 19% and 68%, respectively. In conjunction with each other, the pathogen viability was reduced by 2,965-fold (99.98%), illustrating the prospective use of these two antimicrobials together against mixed species populations.
URI
Go to Link
DOI
http://dx.doi.org/10.1038/s41598-017-14567-7
ISSN
2045-2322
Appears in Collections:
SLS_Journal Papers
Files in This Item:
000414231000016.pdfDownload

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qr_code

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU