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박성훈

Park, Sunghoon
Biochemical Engineering Lab.
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dc.citation.endPage 147 -
dc.citation.startPage 140 -
dc.citation.title JOURNAL OF BIOTECHNOLOGY -
dc.citation.volume 259 -
dc.contributor.author Sankaranarayanan, Mugesh -
dc.contributor.author Somasundar, Ashok -
dc.contributor.author Seol, Eunhee -
dc.contributor.author Chauhan, Ashish Singh -
dc.contributor.author Kwon, Seongjin -
dc.contributor.author Jung, Gyoo Yeol -
dc.contributor.author Park, Sunghoon -
dc.date.accessioned 2023-12-21T21:42:32Z -
dc.date.available 2023-12-21T21:42:32Z -
dc.date.created 2017-08-04 -
dc.date.issued 2017-10 -
dc.description.abstract Biological 3-hydroxypropionic acid (3-HP) production from glycerol is a two-step reaction catalyzed by glycerol dehydratase (GDHt) and aldehyde dehydrogenase (ALDH). Recombinant strains developed for 3-HP production often suffer from the accumulation of a toxic intermediate, 3-hydroxypropionaldehyde (3-HPA). In order to avoid 3-HPA accumulation, balancing of the two enzymatic activities, in the present study, was attempted by employment of synthetic-regulatory cassettes comprising varying-strength promoters and bicistronic ribosome-binding sites (RBSs). When tested in recombinant Escherichia coli, the cassettes could precisely and differentially control the gene expression in transcription, protein expression and enzymatic activity. Five recombinant strains showing different expressions for GDHt were developed and studied for 3-HPA accumulation and 3-HP production. It was found that 3-HPA accumulation could be completely abolished when expressing ALDH at a level approximately 8-fold higher than that of GDHt. One of the strains, SP4, produced 625 mM (56.4 g/L) of 3-HP in a fed-batch bioreactor, though late-period production was limited by acetate accumulation. Overall, this study demonstrated the importance of pathway balancing in 3-HP production as well as the utility of the synthetic cassette architecture for precise control of bacterial gene expression. -
dc.identifier.bibliographicCitation JOURNAL OF BIOTECHNOLOGY, v.259, pp.140 - 147 -
dc.identifier.doi 10.1016/j.jbiotec.2017.07.027 -
dc.identifier.issn 0168-1656 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/22866 -
dc.identifier.url http://www.sciencedirect.com/science/article/pii/S0168165617315523?via%3Dihub -
dc.identifier.wosid 000412035000022 -
dc.language 영어 -
dc.publisher ELSEVIER SCIENCE BV -
dc.title Production of 3-hydroxypropionic acid by balancing the pathway enzymes using synthetic cassette architecture -
dc.type Article -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -

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