BROWSE

Related Researcher

Author

Park, Sunghoon
Research Interests
  • Chemical Engineering

ITEM VIEW & DOWNLOAD

Production of 3-hydroxypropionic acid by balancing the pathway enzymes using synthetic cassette architecture

Cited 0 times inthomson ciCited 0 times inthomson ci
Title
Production of 3-hydroxypropionic acid by balancing the pathway enzymes using synthetic cassette architecture
Author
Sankaranarayanan, MugeshSomasundar, AshokSeol, EunheeChauhan, Ashish SinghKwon, SeongjinJung, Gyoo YeolPark, Sunghoon
Keywords
3-hydroxypropionic acid; Balancing enzymes; Synthetic cassette architecture; 3-HPA; 1,3-PDO
Issue Date
201710
Publisher
ELSEVIER SCIENCE BV
Citation
JOURNAL OF BIOTECHNOLOGY, v.259, no., pp.140 - 147
Abstract
Biological 3-hydroxypropionic acid (3-HP) production from glycerol is a two-step reaction catalyzed by glycerol dehydratase (GDHt) and aldehyde dehydrogenase (ALDH). Recombinant strains developed for 3-HP production often suffer from the accumulation of a toxic intermediate, 3-hydroxypropionaldehyde (3-HPA). In order to avoid 3-HPA accumulation, balancing of the two enzymatic activities, in the present study, was attempted by employment of synthetic-regulatory cassettes comprising varying-strength promoters and bicistronic ribosome-binding sites (RBSs). When tested in recombinant Escherichia coli, the cassettes could precisely and differentially control the gene expression in transcription, protein expression and enzymatic activity. Five recombinant strains showing different expressions for GDHt were developed and studied for 3-HPA accumulation and 3-HP production. It was found that 3-HPA accumulation could be completely abolished when expressing ALDH at a level approximately 8-fold higher than that of GDHt. One of the strains, SP4, produced 625 mM (56.4 g/L) of 3-HP in a fed-batch bioreactor, though late-period production was limited by acetate accumulation. Overall, this study demonstrated the importance of pathway balancing in 3-HP production as well as the utility of the synthetic cassette architecture for precise control of bacterial gene expression.
URI
Go to Link
DOI
http://dx.doi.org/10.1016/j.jbiotec.2017.07.027
ISSN
0168-1656
Appears in Collections:
ECHE_Journal Papers

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qr_code

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU