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DC Field | Value | Language |
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dc.citation.endPage | 9229 | - |
dc.citation.number | 66 | - |
dc.citation.startPage | 9226 | - |
dc.citation.title | CHEMICAL COMMUNICATIONS | - |
dc.citation.volume | 53 | - |
dc.contributor.author | Kang, Myeong-Gyun | - |
dc.contributor.author | Lee, Hakbong | - |
dc.contributor.author | Kim, Beom Ho | - |
dc.contributor.author | Dunbayev, Yerkin | - |
dc.contributor.author | Seo, Jeong Kon | - |
dc.contributor.author | Lee, Changwook | - |
dc.contributor.author | Rhee, Hyun-Woo | - |
dc.date.accessioned | 2023-12-21T22:06:26Z | - |
dc.date.available | 2023-12-21T22:06:26Z | - |
dc.date.created | 2017-08-26 | - |
dc.date.issued | 2017-08 | - |
dc.description.abstract | Alkyl halides are potentially mutagenic carcinogens. However, no efficient fluorescent sensor for alkyl halide detection in human-derived samples has been developed to date. Herein, we report a new protein-based fluorescent sensor for alkyl halides. Analysis of the HaloTag holo-crystal structure with its covalently attached ligand revealed an unexpected cavity, allowing for the design of a new fluorogenic ligand. This ligand showed the highest fluorescence response (300-fold) and fastest binding kinetics (t1/2 < 150 s) to a HaloTag mutant (M175P) protein. This protein-based sensor system was effectively used to detect alkyl halides in human serum and monitor real-time protein alkylation. | - |
dc.identifier.bibliographicCitation | CHEMICAL COMMUNICATIONS, v.53, no.66, pp.9226 - 9229 | - |
dc.identifier.doi | 10.1039/c7cc03714g | - |
dc.identifier.issn | 1359-7345 | - |
dc.identifier.scopusid | 2-s2.0-85027418447 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/22711 | - |
dc.identifier.url | http://pubs.rsc.org/en/Content/ArticleLanding/2017/CC/C7CC03714G#!divAbstract | - |
dc.identifier.wosid | 000407633900010 | - |
dc.language | 영어 | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.title | Structure-guided synthesis of a protein-based fluorescent sensor for alkyl halides | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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