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Takayama, Shuichi
Cell and Microfluidics Lab
Research Interests
  • Bio-MEMS and Microfluidics

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A cost-effective fluorescence mini-microscope for biomedical applications

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Title
A cost-effective fluorescence mini-microscope for biomedical applications
Author
Zhang, Yu ShrikeRibas, JoaoNadhman, AkhtarAleman, JulioSelimovic, SeilaLesher-Perez, Sasha CaiWang, TingManoharan, VijayanShin, Su-RyonDamilano, AlessiaAnnabi, NasimDokmeci, Mehmet RemziTakayama, ShuichiKhademhosseini, Ali
Issue Date
2015
Publisher
ROYAL SOC CHEMISTRY
Citation
LAB ON A CHIP, v.15, no.18, pp.3661 - 3669
Abstract
We have designed and fabricated a miniature microscope from off-the-shelf components and a webcam, with built-in fluorescence capability for biomedical applications. The mini-microscope was able to detect both biochemical parameters, such as cell/tissue viability (e.g. live/dead assay), and biophysical properties of the microenvironment such as oxygen levels in microfabricated tissues based on an oxygen-sensitive fluorescent dye. This mini-microscope has adjustable magnifications from 8-60x, achieves a resolution as high as < 2 mu m, and possesses a long working distance of 4.5 mm (at a magnification of 8x). The mini-microscope was able to chronologically monitor cell migration and analyze beating of microfluidic liver and cardiac bioreactors in real time, respectively. The mini-microscope system is cheap, and its modularity allows convenient integration with a wide variety of pre-existing platforms including, but not limited to, cell culture plates, microfluidic devices, and organs-on-a-chip systems. Therefore, we envision its widespread application in cell biology, tissue engineering, biosensing, microfluidics, and organs-on-chips, which can potentially replace conventional bench-top microscopy where long-term in situ and large-scale imaging/analysis is required.
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DOI
http://dx.doi.org/10.1039/c5lc00666j
ISSN
1473-0197
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SLS_Journal Papers
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