A cost-effective fluorescence mini-microscope for biomedical applications
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- A cost-effective fluorescence mini-microscope for biomedical applications
- Zhang, Yu Shrike; Ribas, Joao; Nadhman, Akhtar; Aleman, Julio; Selimovic, Seila; Lesher-Perez, Sasha Cai; Wang, Ting; Manoharan, Vijayan; Shin, Su-Ryon; Damilano, Alessia; Annabi, Nasim; Dokmeci, Mehmet Remzi; Takayama, Shuichi; Khademhosseini, Ali
- Issue Date
- ROYAL SOC CHEMISTRY
- LAB ON A CHIP, v.15, no.18, pp.3661 - 3669
- We have designed and fabricated a miniature microscope from off-the-shelf components and a webcam, with built-in fluorescence capability for biomedical applications. The mini-microscope was able to detect both biochemical parameters, such as cell/tissue viability (e.g. live/dead assay), and biophysical properties of the microenvironment such as oxygen levels in microfabricated tissues based on an oxygen-sensitive fluorescent dye. This mini-microscope has adjustable magnifications from 8-60x, achieves a resolution as high as < 2 mu m, and possesses a long working distance of 4.5 mm (at a magnification of 8x). The mini-microscope was able to chronologically monitor cell migration and analyze beating of microfluidic liver and cardiac bioreactors in real time, respectively. The mini-microscope system is cheap, and its modularity allows convenient integration with a wide variety of pre-existing platforms including, but not limited to, cell culture plates, microfluidic devices, and organs-on-a-chip systems. Therefore, we envision its widespread application in cell biology, tissue engineering, biosensing, microfluidics, and organs-on-chips, which can potentially replace conventional bench-top microscopy where long-term in situ and large-scale imaging/analysis is required.
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