File Download

There are no files associated with this item.

  • Find it @ UNIST can give you direct access to the published full text of this article. (UNISTARs only)
Related Researcher

박성훈

Park, Sunghoon
Biochemical Engineering Lab.
Read More

Views & Downloads

Detailed Information

Cited time in webofscience Cited time in scopus
Metadata Downloads

Effect of culture medium on fermentative and CO-dependent H-2 production activity in Citrobacter amalonaticus Y19

Author(s)
Ainala, Satish KumarSeol, EunheeKim, Jung RaePark, Sunghoon
Issued Date
2016-05
DOI
10.1016/j.ijhydene.2016.03.088
URI
https://scholarworks.unist.ac.kr/handle/201301/21656
Fulltext
http://www.sciencedirect.com/science/article/pii/S0360319915308697
Citation
INTERNATIONAL JOURNAL OF HYDROGEN ENERGY, v.41, no.16, pp.6734 - 6742
Abstract
Citrobacter amalonaticus Y19 is a unique chemoheterotrophic microorganism capable of carbon monoxide (CO)-dependent hydrogen (H-2) production. To improve its biocatalytic potential, the effects of medium composition on cell growth and H-2 production were investigated. Among several carbon sources tested, maltose resulted in the best CO consumption and H-2 production. Meanwhile, L-cysteine supplementation improved cell growth and H-2 production, suggesting that this amino acid stimulates synthesis of Fe-S clusters. When molybdenum and selenium, which are essential cofactors of formate hydrogen lyase (FHL), were added, formate accumulation was reduced. Optimized medium composition improved the activities of CO-dependent H-2 production (6.3-fold), CODH (22.3-fold) and hydrogenase (2.9-fold). The optimized medium composition also enhanced H-2 production in recombinant C. amalonaticus Y19-cooA, which homologously over expresses CooA, the transcriptional activator of the coo operons. Cells grown under this condition exhibited improved stability in the course of repeated-batch CO-dependent H-2 production under non-growing conditions.
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
ISSN
0360-3199
Keyword (Author)
Citrobacter amalonaticusWater-gas shift reactionCODHCO-HydCooA
Keyword
CARBON-MONOXIDE DEHYDROGENASEHYDROGEN-PRODUCTIONESCHERICHIA-COLIEXPRESSION

qrcode

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.