There are no files associated with this item.
Full metadata record
DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 440 | - |
dc.citation.startPage | 432 | - |
dc.citation.title | BIORESOURCE TECHNOLOGY | - |
dc.citation.volume | 214 | - |
dc.contributor.author | Kumar, Vinod | - |
dc.contributor.author | Durgapal, Meetu | - |
dc.contributor.author | Sankaranarayanan, Mugesh | - |
dc.contributor.author | Somasundar, Ashok | - |
dc.contributor.author | Rathnasingh, Chelladurai | - |
dc.contributor.author | Song, HyoHak | - |
dc.contributor.author | Seung, Doyoung | - |
dc.contributor.author | Park, Sunghoon | - |
dc.date.accessioned | 2023-12-21T23:16:48Z | - |
dc.date.available | 2023-12-21T23:16:48Z | - |
dc.date.created | 2017-02-19 | - |
dc.date.issued | 2016-08 | - |
dc.description.abstract | The current study investigates the impact of mutation of 2,3-butanediol (BDO) formation pathway on glycerol metabolism and 1,3-propanediol (PDO) production by lactate dehydrogenase deficient mutant of Klebsiella pneumoniae J2B. To this end, BDO pathway genes, budA, budB, budC and budO (whole-bud operon), were deleted from K. pneumoniae J2B Delta ldhA and the mutants were studied for glycerol metabolism and alcohols (PDO, BDO) production. Delta budO-mutant-only could completely abolish BDO production, but with reductions in cell growth and PDO production. By modifying the culture medium, the Delta budO mutant could recover its performance on the flask scale. However, in bioreactor experiments, the Delta budO mutant accumulated a significant amount of pyruvate (>73 mM) in the late phase and PDO production stopped concomitantly. Glycolytic intermediates of glycerol, especially glyceraldehyde-3-phosphate (G3P) was highly inhibitory to glycerol dehydratase (GDHt); its accumulation, followed by pyruvate accumulation, was assumed to be responsible for the Delta budO mutant's low PDO production. | - |
dc.identifier.bibliographicCitation | BIORESOURCE TECHNOLOGY, v.214, pp.432 - 440 | - |
dc.identifier.doi | 10.1016/j.biortech.2016.04.032 | - |
dc.identifier.issn | 0960-8524 | - |
dc.identifier.scopusid | 2-s2.0-84964994691 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/21655 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0960852416305193 | - |
dc.identifier.wosid | 000377366900056 | - |
dc.language | 영어 | - |
dc.publisher | ELSEVIER SCI LTD | - |
dc.title | Effects of mutation of 2,3-butanediol formation pathway on glycerol metabolism and 1,3-propanediol production by Klebsiella pneumoniae J2B | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
Tel : 052-217-1404 / Email : scholarworks@unist.ac.kr
Copyright (c) 2023 by UNIST LIBRARY. All rights reserved.
ScholarWorks@UNIST was established as an OAK Project for the National Library of Korea.