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Park, Sunghoon
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  • Chemical Engineering

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Optimum Rebalancing of the 3-Hydroxypropionic Acid Production Pathway from Glycerol in Escherichia coli

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Title
Optimum Rebalancing of the 3-Hydroxypropionic Acid Production Pathway from Glycerol in Escherichia coli
Author
Lim, Hyun GyuNoh, Myung HyunJeong, Jun HongPark, SunghoonJung, Gyoo Yeol
Issue Date
201611
Publisher
AMER CHEMICAL SOC
Citation
ACS SYNTHETIC BIOLOGY, v.5, no.11, pp.1247 - 1255
Abstract
3-Hydroxypropionic acid (3-HP) can be biologically produced from glycerol by two consecutive enzymatic reactions, dehydration of glycerol to 3-hydroxypropionaldehyde (3-HPA) and oxidation of 3-HPA. The pathway has been proved efficient, but imbalance between the rates of the two enzymatic reactions often results in the accumulation of the toxic 3-HPA, which severely reduces cell viability and 3-HP production. In this study, we used UTR engineering to maximally increase the activities of glycerol dehydratase (GDHt) and aldehyde dehydrogenase (ALDH) for the high conversion of glycerol to 3-HP. Thereafter, the activity of GDHt was precisely controlled to avoid the accumulation of 3-HPA by varying expression of dhaB1, a gene encoding a main subunit of GDHt. The optimally balanced E. coil HGL_DBK4 showed a substantially enhanced 3-HP titer and productivity compared with the parental strain. The yield on glycerol, 0.97 g 3HP/g glycerol, in a fed-batch experiment, was the highest ever reported.
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DOI
http://dx.doi.org/10.1021/acssynbio.5b00303
ISSN
2161-5063
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ECHE_Journal Papers
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