File Download

There are no files associated with this item.

  • Find it @ UNIST can give you direct access to the published full text of this article. (UNISTARs only)
Related Researcher

김병수

Kim, Byeong-Su
Read More

Views & Downloads

Detailed Information

Cited time in webofscience Cited time in scopus
Metadata Downloads

Highly efficient gene silencing and bioimaging based on fluorescent carbon dots in vitro and in vivo

Author(s)
Kim, SeongchanChoi, YuriPark, GinamWon, CheolheePark, Young-JoonLee, YounghoonKim, Byeong-SuMin, Dal-Hee
Issued Date
2017-02
DOI
10.1007/s12274-016-1309-1
URI
https://scholarworks.unist.ac.kr/handle/201301/20928
Fulltext
http://link.springer.com/article/10.1007%2Fs12274-016-1309-1
Citation
NANO RESEARCH, v.10, no.2, pp.503 - 519
Abstract
Small interfering RNA (siRNA) is an attractive therapeutic candidate for sequencespecific gene silencing to treat incurable diseases using small molecule drugs. However, its efficient intracellular delivery has remained a challenge. Here, we have developed a highly biocompatible fluorescent carbon dot (CD), and demonstrate a functional siRNA delivery system that induces efficient gene knockdown in vitro and in vivo. We found that CD nanoparticles (NPs) enhance the cellular uptake of siRNA, via endocytosis in tumor cells, with low cytotoxicity and unexpected immune responses. Real-time study of fluorescence imaging in live cells shows that CD NPs favorably localize in cytoplasm and successfully release siRNA within 12 h. Moreover, we demonstrate that CD NP-mediated siRNA delivery significantly silences green fluorescence protein (GFP) expression and inhibits tumor growth in a breast cancer cell xenograft mouse model of tumor-specific therapy. We have developed a multifunctional siRNA delivery vehicle enabling simultaneous bioimaging and efficient downregulation of gene expression, that shows excellent potential for gene therapy.
Publisher
TSINGHUA UNIV PRESS
ISSN
1998-0124
Keyword (Author)
bioimagingcarbon dotgene deliveryRNA interferencetargeted cancer therapy
Keyword
LOW-MOLECULAR-WEIGHTDOUBLE-STRANDED-RNAMESOPOROUS SILICA NANOPARTICLESENDOTHELIAL GROWTH-FACTORHIGH QUANTUM YIELDMAGNETIC NANOPARTICLESFUNCTIONAL DELIVERYGOLD NANOPARTICLESNANODOTS SYNTHESISSIRNA DELIVERY

qrcode

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.