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김용환

Kim, Yong Hwan
Enzyme and Protein Engineering Lab.
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Alginate/bacterial cellulose nanocomposite beads prepared using Gluconacetobacter xylinus and their application in lipase immobilization

Author(s)
Kim, Ji HyunPark, SaeromKim, HyungsupKim, Hyung JooYang, Yung-HunKim, Yong HwanJung, Sang-KyuKan, EunsungLee, Sang Hyun
Issued Date
2017-02
DOI
10.1016/j.carbpol.2016.09.074
URI
https://scholarworks.unist.ac.kr/handle/201301/20648
Fulltext
http://www.sciencedirect.com/science/article/pii/S0144861716311304
Citation
CARBOHYDRATE POLYMERS, v.157, pp.137 - 145
Abstract
Alginate/bacterial cellulose nanocomposite beads, with well-controlled size and regular spherical shapes, were prepared in a simple manner by entrapping Gluconacetobacter xylinus in barium alginate hydrogel beads, followed by cultivation of the entrapped cells in culture media with a low sodium ion concentration. The entire surface of the alginate hydrogel beads containing the cells was covered with cellulose fibers (∼30 nm) after 36 h of cultivation. The cellulose crystallinity index of the alginate/bacterial cellulose beads was 0.7, which was slightly lower than that of bacterial cellulose prepared by cultivating dispersed cells. The water vapor sorption capacity of the alginate/bacterial cellulose beads increased significantly from 0.07 to 38.00 (g/g dry bead) as cultivation time increased. These results clearly indicate that alginate/bacterial cellulose beads have a much higher surface area, crystallinity, and water-holding capacity than alginate beads. The immobilization of lipase on the surface of the nanocomposite beads was also investigated as a potential application of this system. The activity and specific activity of lipase immobilized on alginate/bacterial cellulose beads were 2.6- and 3.8-fold higher, respectively, than that of lipase immobilized on cellulose beads. The alginate/bacterial cellulose nanocomposite beads prepared in this study have several potential applications in the biocatalytic, biomedical, and pharmaceutical fields because of their biocompatibility, biodegradability, high crystallinity, and large surface area.
Publisher
ELSEVIER SCI LTD
ISSN
0144-8617
Keyword (Author)
AlginateBacterial celluloseNanocompositeBeadLipaseImmobilization
Keyword
BACTERIAL CELLULOSEENZYME IMMOBILIZATIONACETOBACTER-XYLINUMALGINATECOMPOSITEETHANOLSCAFFOLDSHANSENIIHYDROGELRELEASE

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