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차채녕

Cha, Chaenyung
Integrative Biomaterials Engineering Lab.
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dc.citation.endPage 8 -
dc.citation.startPage 1 -
dc.citation.title COLLOIDS AND SURFACES B-BIOINTERFACES -
dc.citation.volume 147 -
dc.contributor.author Kim, Suntae -
dc.contributor.author Oh, Jonghyun -
dc.contributor.author Cha, Chaenyung -
dc.date.accessioned 2023-12-21T23:08:45Z -
dc.date.available 2023-12-21T23:08:45Z -
dc.date.created 2016-08-16 -
dc.date.issued 2016-11 -
dc.description.abstract Microfluidic flow-focusing devices (FFD) are widely used to generate monodisperse droplets and microgels with controllable size, shape and composition for various biomedical applications. However, highly inconsistent and often low viability of cells encapsulated within the microgels prepared via microfluidic FFD has been a major concern, and yet this aspect has not been systematically explored. In this study, we demonstrate that the biocompatibility of microfluidic FFD to fabricate cell-laden microgels can be significantly enhanced by controlling the channel geometry. When a single emulsion (“single”) microfluidic FFD is used to fabricate cell-laden microgels, there is a significant decrease and batch-to-batch variability in the cell viability, regardless of their size and composition. It is determined that during droplet generation, some of the cells are exposed to the oil phase which is shown to have a cytotoxic effect. Therefore, a microfluidic device with a sequential (‘double’) flow-focusing channels is employed instead, in which a secondary aqueous phase containing cells enters the primary aqueous phase, so the cells’ exposure to the oil phase is minimized by directing them to the center of droplets. This microfluidic channel geometry significantly enhances the biocompatibility of cell-laden microgels, while maintaining the benefits of a typical microfluidic process. This study therefore provides a simple and yet highly effective strategy to improve the biocompatibility of microfluidic fabrication of cell-laden microgels. -
dc.identifier.bibliographicCitation COLLOIDS AND SURFACES B-BIOINTERFACES, v.147, pp.1 - 8 -
dc.identifier.doi 10.1016/j.colsurfb.2016.07.041 -
dc.identifier.issn 0927-7765 -
dc.identifier.scopusid 2-s2.0-84979658607 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/20235 -
dc.identifier.url http://www.sciencedirect.com/science/article/pii/S0927776516305434 -
dc.identifier.wosid 000384851400001 -
dc.language 영어 -
dc.publisher ELSEVIER SCIENCE BV -
dc.title Enhancing the biocompatibility of microfluidics-assisted fabrication of cell-laden microgels with channel geometry -
dc.type Article -
dc.description.isOpenAccess FALSE -
dc.relation.journalWebOfScienceCategory Biophysics; Chemistry, Physical; Materials Science, Biomaterials -
dc.relation.journalResearchArea Biophysics; Chemistry; Materials Science -
dc.description.journalRegisteredClass scie -
dc.description.journalRegisteredClass scopus -
dc.subject.keywordAuthor Microfluidics -
dc.subject.keywordAuthor Flow-focusing geometry -
dc.subject.keywordAuthor Cell encapsulation -
dc.subject.keywordAuthor Microgel -
dc.subject.keywordAuthor Biocompatibility -
dc.subject.keywordPlus DRUG-DELIVERY APPLICATIONS -
dc.subject.keywordPlus MECHANICAL-PROPERTIES -
dc.subject.keywordPlus COLLOIDAL MICROGELS -
dc.subject.keywordPlus TISSUE CONSTRUCTS -
dc.subject.keywordPlus GELATIN HYDROGEL -
dc.subject.keywordPlus OXIDATIVE STRESS -
dc.subject.keywordPlus SOYBEAN OIL -
dc.subject.keywordPlus ENCAPSULATION -
dc.subject.keywordPlus POLYMERIZATION -
dc.subject.keywordPlus SCAFFOLDS -

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