Full metadata record
DC Field | Value | Language |
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dc.citation.startPage | 24159 | - |
dc.citation.title | SCIENTIFIC REPORTS | - |
dc.citation.volume | 6 | - |
dc.contributor.author | Min, Junseon | - |
dc.contributor.author | Song, Eun Kyung | - |
dc.contributor.author | Kim, Hansol | - |
dc.contributor.author | Kim, Kyoung Taek | - |
dc.contributor.author | Park, Tae Joo | - |
dc.contributor.author | Kang, Sebyung | - |
dc.date.accessioned | 2023-12-22T00:06:21Z | - |
dc.date.available | 2023-12-22T00:06:21Z | - |
dc.date.created | 2016-05-03 | - |
dc.date.issued | 2016-04 | - |
dc.description.abstract | We construct a novel recombinant secondary antibody mimic, GST-ABD, which can bind to the Fc regions of target-bound primary antibodies and acquire multiple HRPs simultaneously. We produce it in tenth of mg quantities with a bacterial overexpression system and simple purification procedures, significantly reducing the manufacturing cost and time without the use of animals. GST-ABD is effectively conjugated with 3 HRPs per molecule on an average and selectively bind to the Fc region of primary antibodies derived from three different species (mouse, rabbit, and rat). HRP-conjugated GST-ABD (HRP-GST-ABD) is successfully used as an alternative to secondary antibodies to amplify target-specific signals in both ELISA and immunohistochemistry regardless of the target molecules and origin of primary antibodies used. GST-ABD also successfully serves as an anchoring adaptor on the surface of GSH-coated plates for immobilizing antigen-capturing antibodies in an orientation-controlled manner for sandwich-type indirect ELISA through simple molecular recognition without any complicated chemical modification. | - |
dc.identifier.bibliographicCitation | SCIENTIFIC REPORTS, v.6, pp.24159 | - |
dc.identifier.doi | 10.1038/srep24159 | - |
dc.identifier.issn | 2045-2322 | - |
dc.identifier.scopusid | 2-s2.0-84964393697 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/19079 | - |
dc.identifier.url | http://www.nature.com/articles/srep24159 | - |
dc.identifier.wosid | 000373763300001 | - |
dc.language | 영어 | - |
dc.publisher | NATURE PUBLISHING GROUP | - |
dc.title | A Recombinant Secondary Antibody Mimic as a Target-specific Signal Amplifier and an Antibody Immobilizer in Immunoassays | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.relation.journalWebOfScienceCategory | Multidisciplinary Sciences | - |
dc.relation.journalResearchArea | Science & Technology - Other Topics | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordPlus | CATALYZED REPORTER DEPOSITION | - |
dc.subject.keywordPlus | PROTEIN CAGE | - |
dc.subject.keywordPlus | EP-CAM | - |
dc.subject.keywordPlus | THERAPY | - |
dc.subject.keywordPlus | CANCER | - |
dc.subject.keywordPlus | DOMAIN | - |
dc.subject.keywordPlus | AMPLIFICATION | - |
dc.subject.keywordPlus | NANOPLATFORM | - |
dc.subject.keywordPlus | EXPRESSION | - |
dc.subject.keywordPlus | ASSAY | - |
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