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Author

Kim, Hajin
Single Molecule Biophysics Lab
Research Interests
  • Biophysics, Single Molecule, fluorescence Spectroscopy, biological Motors, biomolecular assembly

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Direct evidence for sequence-dependent attraction between double-stranded DNA controlled by methylation

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Title
Direct evidence for sequence-dependent attraction between double-stranded DNA controlled by methylation
Author
Yoo, JejoongKim, HajinAksimentiev, AlekseiHa, Taekjip
Keywords
MOLECULAR-DYNAMICS SIMULATIONS; DOUBLE HELICES; BIOGENIC POLYAMINES; CHARGE INVERSION; NUCLEIC-ACIDS; FORCE-FIELD; SYSTEMS; CHROMOSOME; PRECIPITATION; CONDENSATION
Issue Date
201603
Publisher
NATURE PUBLISHING GROUP
Citation
NATURE COMMUNICATIONS, v.7, no., pp.11045 -
Abstract
Although proteins mediate highly ordered DNA organization in vivo, theoretical studies suggest that homologous DNA duplexes can preferentially associate with one another even in the absence of proteins. Here we combine molecular dynamics simulations with single-molecule fluorescence resonance energy transfer experiments to examine the interactions between duplex DNA in the presence of spermine, a biological polycation. We find that AT-rich DNA duplexes associate more strongly than GC-rich duplexes, regardless of the sequence homology. Methyl groups of thymine acts as a steric block, relocating spermine from major grooves to interhelical regions, thereby increasing DNA-DNA attraction. Indeed, methylation of cytosines makes attraction between GC-rich DNA as strong as that between AT-rich DNA. Recent genome-wide chromosome organization studies showed that remote contact frequencies are higher for AT-rich and methylated DNA, suggesting that direct DNA-DNA interactions that we report here may play a role in the chromosome organization and gene regulation
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DOI
http://dx.doi.org/10.1038/ncomms11045
ISSN
2041-1723
Appears in Collections:
SLS_Journal Papers
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