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이세민

Lee, Semin
Computational Biology Lab.
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Characterization of spindle checkpoint kinase mps1 reveals domain with functional and structural similarities to tetratricopeptide repeat motifs of Bub1 and BubR1 checkpoint kinases

Author(s)
Lee, SeminThebault, PhilippeFreschi, LucaBeaufils, SylvieBlundell, Tom L.Landry, Christian RBolanos-Garcia, Victor MElowe, Sabine
Issued Date
2012-02
DOI
10.1074/jbc.M111.307355
URI
https://scholarworks.unist.ac.kr/handle/201301/18890
Fulltext
http://www.jbc.org/content/287/8/5988
Citation
JOURNAL OF BIOLOGICAL CHEMISTRY, v.287, no.8, pp.5988 - 6001
Abstract
Kinetochore targeting of the mitotic kinases Bub1, BubR1, and Mps1 has been implicated in efficient execution of their functions in the spindle checkpoint, the self-monitoring system of the eukaryotic cell cycle that ensures chromosome segregation occurs with high fidelity. In all three kinases, kinetochore docking is mediated by the N-terminal region of the protein. Deletions within this region result in checkpoint failure and chromosome segregation defects. Here, we use an interdisciplinary approach that includes biophysical, biochemical, cell biological, and bioinformatics methods to study the N-terminal region of human Mps1.Wereport the identification of a tandem repeat of the tetratricopeptide repeat (TPR) motif in the N-terminal kinetochore binding region of Mps1, with close homology to the tandem TPR motif of Bub1 and BubR1. Phylogenetic analysis indicates that TPR Mps1 was acquired after the split between deutorostomes and protostomes, as it is distinguishable in chordates and echinoderms. Overexpression of TPR Mps1 resulted in decreased efficiency of both chromosome alignment and mitotic arrest, likely through displacement of endogenous Mps1 from the kinetochore and decreased Mps1 catalytic activity. Taken together, our multidisciplinary strategy provides new insights into the evolution, structural organization, and function of Mps1 N-terminal region. © 2012 by The American Society for Biochemistry and Molecular Biology, Inc
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
ISSN
0021-9258

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