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Kwon, Soon-Yong
Frontier, Innovative Nanomaterials & Devices Lab.
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Establishment of feeder-free culture system for human induced pluripotent stem cell on DAS nanocrystalline graphene

Author(s)
Lee, HyunahNam, DonggyuChoi, Jae KyungAraúzo‐Bravo, Marcos JKwon, Soon-YongZaehres, HolmLee, TaeheePark, Chan YoungKang, Hyun WookHans Robert ShoelerKim, Jeong Beom
Issued Date
2016-02
DOI
10.1038/srep20708
URI
https://scholarworks.unist.ac.kr/handle/201301/18576
Fulltext
http://www.nature.com/articles/srep20708
Citation
SCIENTIFIC REPORTS, v.6, pp.20708
Abstract
The maintenance of undifferentiated human pluripotent stem cells (hPSC) under xeno-free condition requires the use of human feeder cells or extracellular matrix (ECM) coating. However, human-derived sources may cause human pathogen contamination by viral or non-viral agents to the patients. Here we demonstrate feeder-free and xeno-free culture system for hPSC expansion using diffusion assisted synthesis-grown nanocrystalline graphene (DAS-NG), a synthetic non-biological nanomaterial which completely rule out the concern of human pathogen contamination. DAS-NG exhibited advanced biocompatibilities including surface nanoroughness, oxygen containing functional groups and hydrophilicity. hPSC cultured on DAS-NG could maintain pluripotency in vitro and in vivo, and especially cell adhesion-related gene expression profile was comparable to those of cultured on feeders, while hPSC cultured without DAS-NG differentiated spontaneously with high expression of somatic cell-enriched adhesion genes. This feeder-free and xeno-free culture method using DAS-NG will facilitate the generation of clinical-grade hPSC.
Publisher
NATURE PUBLISHING GROUP
ISSN
2045-2322
Keyword
SELF-RENEWALDIFFERENTIATIONFILMSGROWTHLINESSURFACESPLATFORM

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