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DC Field | Value | Language |
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dc.citation.endPage | 26847 | - |
dc.citation.number | 43 | - |
dc.citation.startPage | 26842 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 269 | - |
dc.contributor.author | LEE, Young Han | - |
dc.contributor.author | KIM, Hee Sook | - |
dc.contributor.author | PAI, Jin-Keon | - |
dc.contributor.author | RYU, Sung Ho | - |
dc.contributor.author | Suh, Pann-Ghill | - |
dc.date.accessioned | 2023-12-22T12:43:39Z | - |
dc.date.available | 2023-12-22T12:43:39Z | - |
dc.date.created | 2015-08-13 | - |
dc.date.issued | 1994-10 | - |
dc.description.abstract | The mechanism of phospholipase D (PLD) activation by platelet derived growth factor (PDGF) was examined using a NIH 3T3 fibroblast cell line (3T3-gamma 1) that stably overexpresses PLC-gamma 1 isozyme. Immunoblot analysis revealed that 3T3-gamma 1 cells contained about 10-fold more PLC-gamma 1 than a control cell line (3T3-C) transfected with expression vector lacking PLC-gamma 1 cDNA. PDGF-stimulated PLD activation was 10-fold greater in 3T3-gamma 1 cells than in 3T3-C cells, indicating that PLD activation is dependent upon the level of PLC-gamma 1. Phorbol 12-myristate 13-acetate (PMA) treatment increased PLD activity to a similar extent in both 3T3-gamma 1 cells and control cells. Pretreatment with tyrosine kinase inhibitors including staurosporine and genistein decreased PLD activity by 82.6% and 87.2%, respectively, and completely blocked tyrosine phosphorylation of PDGF receptor and PLC-gamma 1 in 3T3-gamma 1 cells stimulated with PDGF. Moreover, downregulation of protein kinase C by pretreatment of PMA caused complete inhibition of PDGF- and PMA-stimulated PLD activation. Therefore, these results suggest that PDGF-induced PLD activation may be a consequence of primary stimulation of PLC-gamma 1 and that PLD may play a role downstream hom PLC-gamma 1 in PDGF-triggered mitogenesis | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.269, no.43, pp.26842 - 26847 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.scopusid | 2-s2.0-0028034025 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/18556 | - |
dc.identifier.url | http://www.jbc.org/content/269/43/26842.full.pdf+html | - |
dc.identifier.wosid | A1994PQ93100042 | - |
dc.language | 영어 | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.title | ACTIVATION OF PHOSPHOLIPASE-D INDUCED BY PLATELET-DERIVED GROWTH-FACTOR IS DEPENDENT UPON THE LEVEL OF PHOSPHOLIPASE C-GAMMA-1 | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
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