Feasibility of a facile butanol bioproduction using planetary mill pretreatment
Cited 0 times inCited 0 times in
- Feasibility of a facile butanol bioproduction using planetary mill pretreatment
- Kwon, Jeong Heo; Kang, Hyunsoo; Sang, Byoung-In; Kim, Yunje; Min, Jiho; Mitchell, Robert J.; Lee, Jin Hyung
- Planetary mill; Pretreatment; Enhanced bioconversion; Biobutanol; Biofuel
- Issue Date
- ELSEVIER SCI LTD
- BIORESOURCE TECHNOLOGY, v.199, no., pp.283 - 287
- A facile butanol bioproduction process was developed using planetary milling, and Pinus rigida wood waste as a model substrate for fermentable sugars. The use of planetary milling as the pretreatment eliminates the need for washing and transfer of the biomass prior to enzymatic hydrolysis. Moreover, using this pretreatment process resulted in the production of only 0.072 +/- 0.003 g/L soluble phenolic compounds, a concentration that was not inhibitory towards Clostridium beijerinckii NCIMB 8052. As the milling was performed in a compatible buffer (50 mM acetate, pH 4.8), the enzymatic hydrolysis step was initiated by simply adding the cellulase cocktail powder directly to pretreated biomass without washing the biomass or exchanging the buffer, resulting in a glucose yield of 31 g/L (84.02%). Fermentation of the hydrolysate samples by C. beijerinckii NCIMB 8052 gave slightly better butanol yields than cultures grown in a typical lab media (P2), with final concentrations of 6.91 and 6.66 g/L, respectively. (C) 2015 Elsevier Ltd. All rights reserved
Appears in Collections:
- SLS_Journal Papers
- Files in This Item:
- There are no files associated with this item.
can give you direct access to the published full text of this article. (UNISTARs only)
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.