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Mitchell, Robert J.
Applied & Environmental Microbiology Lab (AEML)
Research Interests
  • Pathogens, bdellovibrio bacteriovorus, patho-biotechnology

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Feasibility of a facile butanol bioproduction using planetary mill pretreatment

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Title
Feasibility of a facile butanol bioproduction using planetary mill pretreatment
Author
Kwon, Jeong HeoKang, HyunsooSang, Byoung-InKim, YunjeMin, JihoMitchell, Robert J.Lee, Jin Hyung
Keywords
Planetary mill;  Pretreatment;  Enhanced bioconversion;  Biobutanol;  Biofuel
Issue Date
201601
Publisher
ELSEVIER SCI LTD
Citation
BIORESOURCE TECHNOLOGY, v.199, no., pp.283 - 287
Abstract
A facile butanol bioproduction process was developed using planetary milling, and Pinus rigida wood waste as a model substrate for fermentable sugars. The use of planetary milling as the pretreatment eliminates the need for washing and transfer of the biomass prior to enzymatic hydrolysis. Moreover, using this pretreatment process resulted in the production of only 0.072 +/- 0.003 g/L soluble phenolic compounds, a concentration that was not inhibitory towards Clostridium beijerinckii NCIMB 8052. As the milling was performed in a compatible buffer (50 mM acetate, pH 4.8), the enzymatic hydrolysis step was initiated by simply adding the cellulase cocktail powder directly to pretreated biomass without washing the biomass or exchanging the buffer, resulting in a glucose yield of 31 g/L (84.02%). Fermentation of the hydrolysate samples by C. beijerinckii NCIMB 8052 gave slightly better butanol yields than cultures grown in a typical lab media (P2), with final concentrations of 6.91 and 6.66 g/L, respectively. (C) 2015 Elsevier Ltd. All rights reserved
URI
http://scholarworks.unist.ac.kr/handle/201301/18095
DOI
http://dx.doi.org/10.1016/j.biortech.2015.08.074
ISSN
0960-8524
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