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Author

Mitchell, Robert J.
Applied & Environmental Microbiology Lab (AEML)
Research Interests
  • Pathogens, bdellovibrio bacteriovorus, patho-biotechnology

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Improved Sugar Production by Optimizing Planetary Mill Pretreatment and Enzyme Hydrolysis Process

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Title
Improved Sugar Production by Optimizing Planetary Mill Pretreatment and Enzyme Hydrolysis Process
Author
Kwon, Jeong HeoLee, SiseonLee, Jae-WonHong, Youn-WooChang, Jeong HoSung, DaekyungKim, Sung HyunSang, Byoung-InMitchell, Robert J.Lee, Jin Hyung
Keywords
ETHANOL; CELLULOSE; BIOFUELS; BIOMASS
Issue Date
201508
Publisher
HINDAWI PUBLISHING CORPORATION
Citation
BIOMED RESEARCH INTERNATIONAL, v.2015, no., pp.267538 -
Abstract
This paper describes an optimization of planetary mill pretreatment and saccharification processes for improving biosugar production. Pitch pine (Pinus rigida) wood sawdust waste was used as biomass feedstock and the process parameters optimized in this study were the buffering media, the milling time, the enzyme quantity, and the incubation time. Glucose yields were improved when acetate buffer was used rather than citrate buffer. Initially, with each process variable tests, the optimal values were 100 minutes of milling, an enzyme concentration of 16 FPU/g-biomass, and a 12-hour enzymatic hydrolysis. Typically, interactions between these experimental conditions and their effects on glucose production were next investigated using RSM. Glucose yields from the Pinus rigida waste exceeded 80% with several of the conditions tested, demonstrating that milling can be used to obtain high levels of glucose bioconversion from woody biomass for biorefinery purposes
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DOI
http://dx.doi.org/10.1155/2015/267538
ISSN
2314-6133
Appears in Collections:
SLS_Journal Papers
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