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Author

Cho, Yoon-Kyoung
Integrated Nano-Biotech Lab (INBL)
Research Interests
  • Microfluidics, Lab-on-a-chip, personalized biomedical diagnostics, nanobioengineering

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Rapid and Automated Quantification of Microalgal Lipids on a Spinning Disc

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Title
Rapid and Automated Quantification of Microalgal Lipids on a Spinning Disc
Author
Kim, YubinJeong , Su-NamKim, BolamKim, Dong-PyoCho, Yoon-Kyoung
Keywords
ON-A-DISC; CHLAMYDOMONAS-REINHARDTII; MICROFLUIDIC PLATFORM; EXTRACTION; LAB; BIODIESEL; GROWTH; ACCUMULATION; DEPRIVATION; NITROGEN
Issue Date
201508
Publisher
AMER CHEMICAL SOC
Citation
ANALYTICAL CHEMISTRY, v.87, no.15, pp.7865 - 7871
Abstract
We have developed a fully integrated centrifugal microfluidic device for rapid on-site quantification of lipids from microalgal samples. The fully automated serial process involving cell sedimentation and lysis, liquid-liquid extraction, and colorimetric detection of lipid contents was accomplished within 13 min using a lab-on-a-disc. The presented organic solvent-tolerable (for n-hexane, ethanol) microfluidic disc was newly fabricated by combining thermal fusion bonding and carbon dot-based valving techniques. It is expected that this novel platform will possibly contribute toward sustainable biofuel applications by providing a practical solution for on-site monitoring of lipid accumulation in microalgal samples, thus providing imperative contribution toward energy and environmental purposes of centrifugal microfluidic technology. (Figure Presented). © 2015 American Chemical Society
URI
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DOI
http://dx.doi.org/10.1021/acs.analchem.5b01570
ISSN
0003-2700
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