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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 1901 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1895 | - |
dc.citation.title | JOURNAL OF IMMUNOLOGY | - |
dc.citation.volume | 158 | - |
dc.contributor.author | Seo, Jeong Kon | - |
dc.contributor.author | Choi, Se-Young | - |
dc.contributor.author | Kim, Yong | - |
dc.contributor.author | Baek, Suk Hwan | - |
dc.contributor.author | Kim, Kyong-Tai | - |
dc.contributor.author | Chae, Chi-Bom | - |
dc.contributor.author | Lambeth, J. David | - |
dc.contributor.author | Suh, Pann-Ghill | - |
dc.contributor.author | Ryu, Sung Ho | - |
dc.date.accessioned | 2023-12-22T12:37:52Z | - |
dc.date.available | 2023-12-22T12:37:52Z | - |
dc.date.created | 2015-08-19 | - |
dc.date.issued | 1997-02 | - |
dc.description.abstract | Previously, we identified peptides that stimulate phosphoinositide hydrolysis in several leukocyte cell lines from mixtures of random hexapeptide sequences. Moreover, the peptides activate phospholipase C via a pertusis toxin-sensitive C protein-coupled receptor. We now investigate the structure-activity relationship of the peptides with the goal of improving the activity of the peptides, as well as the biologic function of the peptides. Substitution of the L-methionine at the C terminus of peptides with D-methionine markedly increased the effectiveness of the peptides. The half-maximal effective concentrations of MKYMPm-NH2 and WKYMVm-NH2 for stimulation of phosphoinositide hydrolysis in U266 cells were 30 and 0.5 nM, respectively. By BIAcore analysis we confirmed the existence of a receptor for WKYMVm-NH2. Furthermore, the intracellular calcium concentration increase induced by WKYMVm-NH2 was not inhibited by several chemoattractants (FMLP, IL-8, platelet-activating factor, C5a, granulocyte-macrophage CSF, and granulocyte CSF) suggests that WKYMVm-NH2 has a unique cell surface receptor on leukocytes. WKYMVm-NH2 stimulated the phosphoinositide hydrolysis in U937, HL60, and U266 cells, as well as in human neutrophils. Moreover, WKYMVm-NH2 is more effective than FMLP in the production of superoxide in human neutrophils. The data suggest that WKYMVm-NH2 may have the ability to activate the microbicidal functions of human neutrophils | - |
dc.identifier.bibliographicCitation | JOURNAL OF IMMUNOLOGY, v.158, no.4, pp.1895 - 1901 | - |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.scopusid | 2-s2.0-0031568652 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/16493 | - |
dc.identifier.url | http://www.jimmunol.org/content/158/4/1895.abstract | - |
dc.identifier.wosid | A1997WG30400049 | - |
dc.language | 영어 | - |
dc.publisher | AMER ASSOC IMMUNOLOGISTS | - |
dc.title | A peptide with unique receptor specificity: stimulation of phosphoinositide hydrolysis and induction of superoxide generation in human neutrophils | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
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