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DC Field | Value | Language |
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dc.citation.endPage | 10112 | - |
dc.citation.number | 29 | - |
dc.citation.startPage | 10107 | - |
dc.citation.title | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | - |
dc.citation.volume | 131 | - |
dc.contributor.author | Rhee, Hyun-Woo | - |
dc.contributor.author | Choi, So Jung | - |
dc.contributor.author | Yoo, Sang Ho | - |
dc.contributor.author | Jang, Yong Oh | - |
dc.contributor.author | Park, Hun Hee | - |
dc.contributor.author | Maria Pinto, Rosa | - |
dc.contributor.author | Carlos Cameselle, Jose | - |
dc.contributor.author | Sandova, Francisco J. | - |
dc.contributor.author | Roje, Sanja | - |
dc.contributor.author | Han, Kyungja | - |
dc.contributor.author | Chung, Doo Soo | - |
dc.contributor.author | Suh, Junghun | - |
dc.contributor.author | Hong, Jong-In | - |
dc.date.accessioned | 2023-12-22T07:43:28Z | - |
dc.date.available | 2023-12-22T07:43:28Z | - |
dc.date.created | 2015-07-15 | - |
dc.date.issued | 2009-07 | - |
dc.description.abstract | Flavins, comprising flavin mononucleotide (FMN), flavin adenine dinucleotide (FAD), and riboflavin (RF, vitamin B-2), play important roles in numerous redox reactions such as those taking place in the electron-transfer chains of mitochondria in all eukaryotes and of plastids in plants. A selective chemosensor for flavins would be useful not only in the investigation of metabolic processes but also in the diagnosis of diseases related to flavins; such a sensor is presently unavailable. Herein, we report the first bifunctional chemosensor (PTZ-DPA) for flavins. PTZ-DPA consists of bis(Zn2+-dipicolylamine) and phenothiazine. Bis(Zn2+-dipicolylamine) (referred to here as XyDPA) was found to be an excellent catalyst in the conversion of FAD into cyclic FMN (riboflavin 4',5'-cyclic phosphate, cFMN) under physiological conditions, even at pH 7.4 and 27 degrees C, with less than 1 mol % of substrate. Utilizing XyDPA's superior function as an artificial FMN cyclase and phenothiazine as an electron donor able to quench the fluorescence of an isoalloxazine ring, PTZ-DPA enabled selective fluorescent discrimination of flavins (FMN, FAD, and RF): FAD shows ON(+), FMN shows OFF(-), and RF shows NO(0) fluorescence changes upon the addition of PTZ-DPA. With this selective sensing property, PTZ-DPA is applicable to real-time fluorescent monitoring of riboflavin kinase (RF to FMN), alkaline phosphatase (FMN to RF), and FAD synthetase (FMN to FAD) | - |
dc.identifier.bibliographicCitation | JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, v.131, no.29, pp.10107 - 10112 | - |
dc.identifier.doi | 10.1021/ja9018012 | - |
dc.identifier.issn | 0002-7863 | - |
dc.identifier.scopusid | 2-s2.0-67651207208 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/12123 | - |
dc.identifier.url | http://pubs.acs.org/doi/abs/10.1021/ja9018012 | - |
dc.identifier.wosid | 000268395000058 | - |
dc.language | 영어 | - |
dc.publisher | AMER CHEMICAL SOC | - |
dc.title.alternative | A bifunctional molecule as an artificial flavin mononucleotide cyclase and a chemosensor for selective fluorescent detection of flavins | - |
dc.title | A Bifunctional Molecule as an Artificial Flavin Mononucleotide Cyclase and a Chemosensor for Selective Fluorescent Detection of Flavins | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
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