Detailed Information
Cited time in 
Cited time in 
Cited time in
Metadata Downloads
Enzymatic cleavage of uracil-containing single-stranded DNA linkers for the efficient release of affinity-selected circulating tumor cells
- Author(s)
- Nair, Soumya V., Witek, Malgorzata A., Jackson, Joshua M., Lindell, Maria A. M., Hunsucker, Sally A., Sapp, Travis, Perry, E., Hupert, Mateusz L., Bae-Jump, Victoria, Gehrig, Paola A., Wysham, Weiya Z., Armistead, Paul M., Voorhees, Peter, Soper, Steven A.
- Issued Date
- 2015-02
- Citation
- CHEMICAL COMMUNICATIONS, v.51, no.15, pp.3266 - 3269
- Abstract
- We report a novel strategy to enzymatically release affinity-selected cells, such as circulating tumor cells (CTCs), from surfaces with high efficiency (similar to 90%) while maintaining cell viability (>85%). The strategy utilizes single-stranded DNAs that link a capture antibody to the surfaces of a CTC selection device. The DNA linkers contain a uracil residue that can be cleaved.
- Publisher
- ROYAL SOC CHEMISTRY
- ISSN
- 1359-7345
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.