Redox-degradable biocompatible hyperbranched polyglycerols: Synthesis, copolymerization kinetics, degradation, and biocompatibility
- Author(s)
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Son, Suhyun, Shin, Eeseul, Kim, Byeong-Su
- Issued Date
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2015-02
- DOI
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10.1021/ma502242v
- URI
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https://scholarworks.unist.ac.kr/handle/201301/10763
- Fulltext
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http://pubs.acs.org/doi/abs/10.1021/ma502242v
- Citation
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MACROMOLECULES, v.48, no.3, pp.600 - 609
- Abstract
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Polymers that are biocompatible and degrade in response to stimuli are highly desirable as smart drug-delivery carriers. We report the first novel redox-degradable hyperbranched polyglycerols. A glycerol monomer containing a disulfide bond, i.e., 2-((2-(oxiran-2-ylmethoxy)ethyl)disulfanyl)ethan-1-ol (SSG), was designed and polymerized through anionic ring-opening multibranching polymerization to yield a series of redox-degradable hyperbranched polyglycerols (PSSGs) with controlled molecular weights (2000-11 000 g/mol) and relatively low molecular weight distributions (Mw/Mn < 1.15). In addition, copolymerization with a nondegradable glycerol (G) monomer provided P(G-co-SSG) copolymers, which contained an adjustable fraction of degradable moieties within their polyglycerol backbones. The polymerization was characterized using 1H and 13C NMR spectroscopy, GPC, and MALDI-ToF mass spectrometry. The copolymerization process was also evaluated using quantitative in situ 13C NMR kinetic measurements in bulk, which revealed that the reaction kinetics of G were faster than those of the SSG monomer, leading to a gradient during the copolymerization process. Furthermore, we explored the redox-responsive degradation of the polymers upon treatment with a reducing agent, which resulted in selective degradation of the polymers in small segments. In vitro cytotoxicity studies, such as MTT and CCK-8 assays, revealed the superior biocompatibility of these new polymers even at high concentrations of 500 μg/mL. We anticipate that these novel redox-degradable and highly biocompatible polyglycerols will find applications in a variety of emerging biomedical fields.
- Publisher
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AMER CHEMICAL SOC
- ISSN
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0024-9297
- Keyword
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CROSS-LINKED MICELLES, BLOCK-COPOLYMERS, MULTIFUNCTIONAL POLYETHERS, SIDE-CHAINS, GLUTATHIONE, RELEASE, DERIVATIVES, DENDRIMERS, POLYMERS, SMART
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